Postdoctoral Fellow - Dr. Yiliang Chen - Transfusion Medicine, Vascular Biology & Cell Therapy

Located in Milwaukee, Wisconsin, the Versiti Blood Research Institute (VBRI) is the largest blood-focused research institute in the United States. With over 30 basic, translational, and clinical investigators, the institute is dedicated to advancing scientific knowledge and developing innovative therapies related to blood and blood disorders. The institute's research interests encompass a wide range of topics, including transfusion medicine, cellular therapy, thrombosis/hemostasis, immunology, and hematologic malignancies. As a vital part of Versiti, a non-profit organization with a blood bank at its core, the VBRI strives to improve lives through innovative research and the development of novel diagnostics and treatments.

VBRI is part of the Milwaukee Regional Medical Campus, a rapidly growing clinical-translational research hub. VBRI's proximity to the Medical College of Wisconsin, Childrens' Hospital of Wisconsin, and Froedtert Hospital facilitates seamless interdisciplinary collaborations. A range of exemplary core facilities support highly competitive research. In the summer of 2024, construction of a new research building began, doubling existing VBRI research space to enable the recruitment of 10-15 new faculty. With its renowned researchers, strategic expansion plans and location in a highly collaborative environment, the VBRI is poised to maintain and expand its position as one of the leading hematology institutes in the world.

Under the direction of a Principal Investigator, further develops and expands understanding of scientific theory in his/her area of interest. Incumbent also learns and applies scientific laboratory techniques and gains experience in other areas of research that will lead to increased probability of success as an independent scientist. This is a training position with an expected duration of 1 - 3 years.

We are interested in a chronic inflammatory disease called atherosclerosis, which is the leading cause of death in the developed countries. Atherosclerosis is characterized by atherosclerotic plaques in the medium and large arteries and the major components of the plaques are lipid-laden innate immune cells called macrophages. Using a variety of genetically modified mice as in vivo animal models together with in vitro cell culture model and many biochemical techniques, we study the molecular mechanisms underlying the pro-atherogenic functions of the macrophages.

We are also using novel state-of-the-art technologies including single cell RNA sequencing, high resolution confocal microscopy, Seahorse extracellular flux metabolic assays, GC-MS, and so on to explore cell expression network that are linked to immune activation and metabolic status. With these novel tools, we are able to study not only the macrophage functions but also the cross talk between macrophage and other cell types.

MAJOR RESPONSIBILITIES * Works with Principal Investigator to develop a research project. * Executes laboratory experiments specific to aims of research. * Troubleshoots assays and customizes experimental tools to answer research questions. * Coordinates external and internal resources necessary for completion of research plan. * Provides direction to Technologists and Trainees. * Generates clear and complete records of experimental work. * Communicates results of research at meetings. * Participates in publication and presentation of research.
• GENERAL DUTIES * Adheres to BCW, Bio-safety, NRC, OSHA procedures and regulations. * Attends required laboratory meetings. * Maintains currency with information relative to the field of research being performed. * Serves as a resource for techniques, information, instrumentation, interpretation of data, etc. for laboratory personnel * May be expected to help direct the work of Research Technologists. * Operates, calibrates and performs routine maintenance on specialized research equipment. * Prepares solutions, reagents, media or stains necessary for experimental procedures. * May maintain or oversee maintenance of inventory of laboratory supplies and equipment. * May prepare and/or authorize requisitions for ordering supplies and equipment. * Performs other laboratory duties as assigned.
• Performs other duties as assigned
• Complies with all policies and standards

Education

• PhD required
• equivalent degree required

Experience

• 0 - 6 years of postdoctoral training required

Knowledge, Skills and Abilities

• Demonstrated ability to independently apply understanding of scientific theory to the structure and execution of research experiments. required
• Demonstrated ability to interpret and explain the theoretical basis of the work performed. required
• Ability to perform research procedures independently. required
• Immediate proficiency in basic and sophisticated experimental tests and procedures. required
• Exceptional organizational skills. required
• Strong detail orientation and analytical ability. required
• Excellent problem solving skills. required
• Ability to prioritize and execute multiple tasks and meet deadlines. required
• Computer literate with an emphasis on graphics, graphing, data management, and statistical applications required

Tools and Technology

• Specialized research equipment required
• Personal computer required

#LI-EH1

#VBRI